The adapted “single-section” Golgi method and the DiI fluorescent dye in microscopy for describing neuronal and glial morphology in different species
نویسندگان
چکیده
Department of Basic Sciences/Physiology, Federal University of Health Sciences, R. Sarmento Leite 245, Porto Alegre RS 90050-110, Brazil Graduate Course in Pathology, Federal University of Health Sciences, R. Sarmento Leite 245, Porto Alegre RS 90050110, Brazil Graduate Course in Neuroscience, Institute of Basic Sciences, Federal University of Rio Grande do Sul, R. Sarmento Leite 500, Porto Alegre RS 90050-110, Brazil Department of Natural Sciences, Federal University of São João del-Rei, Praça Frei Orlando 170, São João del-Rei MG 36307-352, Brazil Laboratory of Synaptic Structure, Department of Cell/Molecular Biology and Biopathogens, Graduate Courses in Neuroscience & Behavior and in Cell and Molecular Biology, University of São Paulo, Ribeirão Preto School of Medicine, Av. Bandeirantes 3900, Ribeirão Preto SP 14049-900, Brazil
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DiOlistic Labeling of Neurons in Tissue Slices: A Qualitative and Quantitative Analysis of Methodological Variations
Fine neuronal morphology, such as dendritic spines, classically has been studied using the Golgi technique; however, Golgi staining is difficult to combine with other histological techniques. With the increasing popularity of fluorescent imaging, a number of fluorescent dyes have been developed that enable the coupling of multiple fluorescent labels in a single preparation. These fluorescent dy...
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